期刊
LAB ON A CHIP
卷 17, 期 2, 页码 235-240出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6lc01305h
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资金
- Ministry of Science and Technology of China [2015AA0200601, 2016YFC0900100]
- National Natural Science Foundation of China [21327808, 21525521]
Stable water-in-oil emulsion is essential to digital PCR and many other bioanalytical reactions that employ droplets as microreactors. We developed a novel technology to produce monodisperse emulsion droplets with high efficiency and high throughput using a bench-top centrifuge. Upon centrifugal spinning, the continuous aqueous phase is dispersed into monodisperse droplet jets in air through a micro-channel array (MiCA) and then submerged into oil as a stable emulsion. We performed dPCR reactions with a high dynamic range through the MiCA approach, and demonstrated that this cost-effective method not only eliminates the usage of complex microfluidic devices and control systems, but also greatly suppresses the loss of materials and cross-contamination. MiCA-enabled highly parallel emulsion generation combines both easiness and robustness of picoliter droplet production, and breaks the technical challenges by using conventional lab equipment and supplies.
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