期刊
WORLD JOURNAL OF MENS HEALTH
卷 37, 期 2, 页码 186-198出版社
KOREAN SOC SEXUAL MEDICINE & ANDROLOGY
DOI: 10.5534/wjmh.180041
关键词
Germ cells; Leydig cells; Testicular diseases; Testosterone
资金
- National Natural Science Foundation of China [81660535]
- Project of Science and Technology of Jiangxi Provincial Education Department [GJJ170966]
- Doctoral Research Start-Up Foundation of Jiujiang University [8879522]
Purpose: This study investigated the role of natriuretic peptide receptor 2 (NPR2) on cell proliferation and testosterone secretion in mouse Leydig cells. Materials and Methods: Mouse testis of different postnatal stages was isolated to detect the expression C-type natriuretic peptide (CNP) and its receptor NPR2 by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Leydig cells isolated from mouse testis were cultured and treated with shNPR2 lentiviruses or CNP. And then the cyclic guanosine monophosphate production, testosterone secretion, cell proliferation, cell cycle and cell apoptosis in mouse Leydig cells were analyzed by ELISA, RT-qPCR, Cell Counting Kit-8, and flow cytometry. Moreover, the expression of NPR2, cell cycle, apoptosis proliferation and cell cycle related gene were detected by RT-qPCR and Western blot. Results: Knockdown of NPR2 by RNAi resulted in S phase cell cycle arrest, cell apoptosis, and decreased testosterone secretion in mouse Leydig cells. Conclusions: Our study provides more evidences to better understand the function of CNP/NPR2 pathway in male reproduction, which may help us to treat male infertility.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据