期刊
CHEMICAL SCIENCE
卷 10, 期 14, 页码 4032-4037出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8sc05588b
关键词
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资金
- Deutsche Forschungsgemeinschaft [Wa 1386/15-2]
- KIT
Two different and small functions for inverse electron demand Diels-Alder reactions were applied for dual labeling of DNA: the 1,2,4-triazine was attached to the 5-position of 2-deoxyuridine triphosphate, and the 1-methylcyclopropene to the 7-position of 7-deaza-2-deoxyadenosine triphosphate. These two modified nucleotides were sequence-selectively incorporated into oligonucleotides by DNA polymerases. These products were labeled by two different fluorescent dyes using postsynthetic reactions that are not only bioorthogonal in general, but also mutually orthogonal.
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