期刊
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC
卷 30, 期 5, 页码 357-362出版社
AKADEMIAI KIADO RT
DOI: 10.1556/1006.2017.30.5.4
关键词
Salvia officinalis L.; Thin-layer chromatography-direct bioautography; Antibacterial activity; Bioassay; Liquid chromatography-quadrupole time-of-flight mass spectrometry
资金
- Hungarian Scientific Research Fund (OTKA) [PD83487]
- Bolyai grant
- [POPW.01.03.00-06-003/09-00]
Thin-layer chromatography-direct bioautography (TLC-DB) followed by liquid chromatography. tandem mass spectrometry (LC. MS/MS) was used for screening and tentative identification of the antibacterial constituents of Salvia officinalis L. ethanol extract. Seven bacterial strains were used as test organisms, both pathogenic and nonpathogenic, that is, Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Staphylococcus epidermidis, Micrococcus luteus, Bacillus subtilis, luminescence gene-tagged Pseudomonas syringae pv. maculicola, and naturally luminescent marine bacterium Aliivibrio fischeri. Eight fractions with the widest antimicrobial spectrum were detected using TLC-DB, isolated by semi-preparative TLC, and subjected to LC-MS/MS analyses. Finally, five bioactive components were tentatively identified, based on their fragmentation pattern, such as salvigenin, cirsimaritin, rosmanol, carnosic acid, and 12-O-methyl carnosic acid.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据