期刊
JOURNAL OF VIROLOGICAL METHODS
卷 246, 期 -, 页码 8-14出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2017.03.011
关键词
Ebolavirus; Ebola virus disease; Diagnosis; RT-LAMP
资金
- Ministry of Health, Labor, and Welfare of Japan
- Japan Agency for Medical Research and Development (AMED) [16fk0108319s0203]
- Grants-in-Aid for Scientific Research [15H01249] Funding Source: KAKEN
Ebola virus disease (EVD), a highly virulent infectious disease caused by ebolaviruses, has a fatality rate of 25-90%. Without a licensed chemotherapeutic agent or vaccine for the treatment and prevention of EVD, control of outbreaks requires accurate and rapid diagnosis of cases. In this study, five sets of six oligonucleotide primers targeting the nucleoprotein gene were designed for specific identification of each of the five ebolavirus species using reverse transcription-loop mediated isothermal amplification (RT-LAMP) assay. The detection limits of the ebolavirus species-specific primer sets were evaluated using in vitro transcribed RNAs. The detection limit of species-specific RT-LAMP assays for Zaire ebolavirus, Sudan ebolavirus, Tat Forest ebolavirus, and Bundibugyo ebolavirus was 256 copies/reaction, while the detection limit for Reston ebolavirus was 64 copies/reaction, and the detection time for each of the RT-LAMP assays was 13.3 +/- 3.0, 19.8 +/- 4.6, 14.3 +/- 0.6, 16.1 +/- 4.7, and 19.8 +/- 2.4 min (mean +/- SD), respectively. The sensitivity of the species-specific RT-LAMP assays were similar to that of the establishedRT-PCR and quantitative RT-PCR assays for diagnosis of EVD and are suitable for field or point-of-care diagnosis. The RT-LAMP assays were specific for the detection of the respective species of ebolavirus with no cross reaction with other species of ebolavirus and other viral hemorrhagic fever viruses such as Marburg virus, Lassa fever virus, and Dengue virus. The species-specific RT-LAMP assays developed in this study are rapid, sensitive, and specific and could be useful in case of an EVD outbreak.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据