期刊
JOURNAL OF VIROLOGICAL METHODS
卷 249, 期 -, 页码 165-169出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2017.09.011
关键词
Goose parvovirus; Novel goose parvovirus-related virus; Short beak and dwarfism syndrome; Duplex semi-nested PCR assay; Tissue tropism
资金
- National Key R D Program [2016YFD0500800]
- Funds of Shandong Double Tops Program, China [SYL2017YSTD11]
- Project of Shandong Province Agriculture Major Applied Technique Innovation, China
- Shandong Modern Agricultural Technology & Industry System, China [SDAIT-11-15]
Duck short beak and dwarfism syndrome (SBDS) is an emerging infectious disease caused by a novel goose parvovirus-related virus (NGPV) in China. Until now, it remains uncertain whether the Cherry Valley ducks and mule ducks with SBDS are co-infected with classical goose parvovirus (GPV) and NGPV. In this study, a duplex semi-nested PCR assay with high specificity and sensitivity was developed for detection of the two viruses. Using the duplex PCR assay, NGPV was tested positive in all the 15 duck flocks with SBDS, whereas classical GPV was not detected in all the 133 sick and dead ducks collected from East China. A total of 87 (91.58%) Cherry Valley ducks aged from 5 to 18 days and 35 (92.11%) mule ducks aged from 17 to 25 days were detected positive for NGPV. In the NGPV-positive ducks, the virus detection rates were 81.97% to 8.20% in heart, liver, spleen, lung, kidney, pancreas, bile, thymus, bursa of Fabricius, and brain. The results indicated that NGPV was prevalent in the duck flocks of East China, whereas classical GPV was not detected in Cherry Valley ducks and mule ducks with SBDS. NGPV has extensive tissue tropism in Cherry Valley duck and mule duck, which could invade both the central and peripheral immune organs and break through the blood-brain barrier of ducks.
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