期刊
EXPRESS POLYMER LETTERS
卷 13, 期 7, 页码 632-641出版社
BUDAPEST UNIV TECHNOL & ECON
DOI: 10.3144/expresspolymlett.2019.53
关键词
biocompatible polymers; electrospinning; nanotomography; tissue spheroids; bioprinting
资金
- Russian Scientific Foundation [17-75-10098]
- Russian Science Foundation [17-75-10098] Funding Source: Russian Science Foundation
We present a 3D study of nanostructural features of a bioprinted tissue spheroid interacting with polyurethane dual-scale biocompatible scaffold manufactured by three-dimensional printing and electrospinning. Three-dimensional analysis of fibroblasts interacting with electrospun polyurethane fibers was conducted using scanning probe nanotomography with an experimental setup combining ultramicrotome and a scanning probe microscope. Three-dimensional reconstruction demonstrates direct visualization of cell membrane protrusions and coherent cell-fiber interfaces, the formation of which is a prerequisite for an efficient tissue engineered implant. Analysis of obtained 3D data allows for quantitative calculation of the important morphological parameters of adhered cells, scaffolds, and cell-scaffold interfaces. The proposed method may be successfully applied to investigate 3D cell-scaffold constructs at nanoscale.
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