Development of a two-photon fluorescent probe for the selective detection of β-galactosidase in living cells and tissues

The development of two-photon bio-imaging probes for trapping specific enzyme activities in overexpressed tissue is very practical and significant. In this work, we developed a two-photon fluorescent b-gal probe G-GAL, which was made with GCTPOC as the fluorescence reporter and a b-galactosidase cleavable crew as the enzyme activity initiator. The purpose of designing this probe was to detect b-galactosidase in living cells and tissues. The probe G-GAL has the advantage of rapid response, great fluorescence enhancement and superior specificity. Furthermore, the probe has excellent tissue penetration.