期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
卷 15, 期 7, 页码 1368-1382出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.33233
关键词
exosome; hair follicle stem cells; dermal papilla; proliferation
资金
- National Natural Science Foundation of China [31772571, 31572369, 31872332, 31760650]
- Key Research Program of Shaanxi Province [2017NY-072]
- China Agriculture Research System [CARS-39]
Recent studies have demonstrated that dermal papilla cell-derived exosomes (DPC-Exos) promote the anagen stage of hair follicle (HF) growth and delay the catagen stage. However, the roles of DPC-Exos in regulating hair follicle stem cell (HFSC) quiescence and activation remain unknown. Here, we found that HFSC differentiation was induced by co-culture with DPCs, and that DPC-Exos attached to the surface of HFSCs. Using micro RNA (miRNA) high-throughput sequencing, we identified 111 miRNAs that were significantly differentially expressed between DPC-Exos and DPCs, and the predicted target genes of the top 34 differentially expressed miRNAs indicated that DPC-Exos regulate HFSCs proliferation and differentiation via genes involved in cellular signal transduction, fatty acid expression regulation, and cellular communication. The overexpression of miR-22-5p indicated that it negatively regulates HFSC proliferation and LEFI was revealed as the direct target gene of miR-22-5p. We therefore propose the miR-22-5p-LEFI axis as a novel pathway regulating HFSC proliferation.
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