期刊
ACS APPLIED MATERIALS & INTERFACES
卷 11, 期 25, 页码 22074-22084出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b00845
关键词
stem cell; controlled differentiation; real-time monitoring cell differentiation; up-conversion nanoparticles; aggregation-induced emission
资金
- Health and Medical Research Fund
- Food and Health Bureau
- Government of the Hong Kong Special Administrative Region [02133356]
- Chow Yuk Ho Technology Centre for Innovative Medicine (The Chinese University of Hong Kong)
- National Basic Research Program of China (973 Program) [2013CB834701, 2013C834702]
- University Grants Committee of Hong Kong [AoE/P-03/08]
- Research Grants Council of Hong Kong [16301614, 16305015, N_HKUST604/14]
- Innovation and Technology Commission [ITC-CNERC14SC01, RE:ITCPD/17-9]
- Guangdong Innovative Research Team Program [201101C0105067115]
Controlling the differentiation of stem cells and monitoring cell differentiation has attracted much research interest since the discovery of stem cells. In this regard, a novel near-infrared (NIR) light-activated nanoplatform is obtained by encapsulating the photoactivatable caged compound (DMNPE/siRNA) and combining a MMP13 cleaved imaging peptide-tetrapheny-lethene (TPE) unit conjugated with the mesoporous silica-coated up-conversion nanoparticles (UCNPs) for the remote control of cell differentiation and, simultaneously, for the real-time monitoring of differentiation. Upon NIR light illumination, the photoactivated caged compound is activated, and the siRNA is released from UCNPs, allowing controlled differentiation of stem cells by light. More importantly, MMP13 enzyme triggered by osteogenic differentiation would effectively cleave the TPE probe peptide, thereby allowing the real-time monitoring of differentiation in living stem cells by aggregation-induced emission (AIE).
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