期刊
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
卷 12, 期 3, 页码 583-597出版社
WILEY
DOI: 10.1002/term.2474
关键词
emulsion electrospinning; growth factor; needleless core; shell electrospinning; Pluronic F-68; time-regulated release
类别
资金
- Grant Agency of Charles University [1262414, 512216]
- Czech Science Foundation [15-15697S]
- Ministry of Education, Youth, and Sports of the Czech Republic [NPU I:LO1508, NPU I:LO1309]
- Ministry of Interior of the Czech Republic (programme BV III/1-VS) [VI20152018010]
- Czech Health Research Council [15-33094A, 16-29680A, 16-28637A]
- University Hospital Motol [9775]
In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process. The nanofibres are based on poly-epsilon-caprolactone (PCL) as a continuous phase combined with a droplet phase based on Pluronic F-68 (PF-68). The PCL-PF-68 nanofibres show a time-regulated release of active molecules. Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5-(4,6-dichlorotriazinyl) aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor- and basic fibroblast growth factor]. In addition, the PF-68 facilitates the preservation of the bioactivity of delivered proteins. The system's potential was highlighted by an improvement in the metabolic activity and proliferation of mesenchymal stem cells. The developed system has the potential to deliver susceptible molecules in tissue-engineering applications.
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