期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 81, 期 5, 页码 787-792出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.19-0009
关键词
BLV; bovine leukemia virus; enzootic bovine leucosis; fLAMP; fluorescent loopmediated isothermal amplification
资金
- JSPS KAKENHI [JP18K05535]
- Japan Racing Horse Health Association
Bovine leukemia virus (BLV) causes enzootic bovine leukosis (EBL), a condition that threatens the sustainability of the livestock industry. A fluorescent loop-mediated isothermal amplification (fLAMP) assay targeting BLV env sequences was developed and used to evaluate 100 bovine blood samples. Compared with a conventional real-time PCR (rPCR) assay, the fLAMP assay achieved 87.3% (62/71) sensitivity and 100% (29/29) specificity. The rPCR assay took 65 min, while the fLAMP assay took 8 min to 30 min from the beginning of DNA amplification to final judgement with a comparable limit of detection. The fLAMP is a potential tool for the rapid and simple diagnosis of BLV infection to supplement ELISA testing and can be used by local laboratories and slaughterhouses without special equipment.
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