A ratiometric fluorescent probe for hydrogen sulfide based on the nucleophilic substitution-cyclization of diselenides

A new ratiometric fluorescence probe for hydrogen sulfide is developed. The design was based on a H2S-mediated diselenide cleavage and intramolecular cyclization to release the fluorophore. The probe showed high selectivity and sensitivity for H2S over other reactive sulfur species and was successfully applied for the detection of H2S in bovine plasma and bioimaging in live cells. (C) 2015 Elsevier Ltd. All rights reserved.