期刊
BIO-PROTOCOL
卷 9, 期 12, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.3280
关键词
Dengue virus (DENV); Virus-like particle (VLP); COS-1 cell; VLP purification; Sucrose gradient centrifugation
类别
资金
- Ministry of Science and Technology Taiwan [MOST-107-2313-B-005-038-MY3, MOST-104-2321-B-005-006]
Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires abundant, and high-purity production methods. Here, we describe a robust protocol for producing DENV VLPs from transiently-transformed or stable COS-1 cells and further provide an easily adaptable antigen purification method by sucrose gradient centrifugation.
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