期刊
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS
卷 34, 期 5, 页码 1004-1015出版社
OPTICAL SOC AMER
DOI: 10.1364/JOSAB.34.001004
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资金
- Japan Agency for Medical Research and Development (AMED)
- Grants-in-Aid for Scientific Research [15H02117] Funding Source: KAKEN
We present a single-beam coherent Raman microscopy method based on pump-probe, time-resolved stimulated Raman scattering (SRS) measurements with shaped probe pulses. In the single-beam method, we divide a broad-band laser spectrum into three frequency bands for the pump, phase-modulated (PM) probe, and local oscillator (LO) probe pulses. Multiple low-wavenumber Raman modes are efficiently excited by an impulsive pump pulse, and a specific Raman mode can be selectively probed using temporal beam coupling between the PM and LO probe pulses via the Raman-induced refractive index modulation. To achieve both high sensitivity and a high spectral resolution, we allocate a large spectral bandwidth (164 cm(-1)) to two probe bands and use a new selective detection scheme based on the spectral focusing technique. By giving a strong group delay dispersion to the probes (45000 fs(2)), we can obtain an improved spectral resolution of down to 25 cm-1. In a proof-of-concept experiment, the intrinsic molecular-vibration contrast of sevoflurane, an inhaled anesthetic drug, is successfully visualized. This result suggests that single-beam SRS imaging with pulse shaping is a potentially powerful tool for detecting the Raman signals of small-molecule drugs in living cells and tissues. (C) 2017 Optical Society of America
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