期刊
CANCER GENOMICS & PROTEOMICS
卷 16, 期 4, 页码 273-286出版社
INT INST ANTICANCER RESEARCH
DOI: 10.21873/cgp.20132
关键词
Prostate cancer; metastasis; mutated peptides; quantitative proteomics; proteogenomics
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Korean government (MSIT) [2014M3A9D3034164, 2015R1A2A2A01004286, 2016R1C1B1011180, 2019R1A2C100 4046]
- National Research Foundation of Korea [2014M3A9D3034164, 2016R1C1B1011180, 2015R1A2A2A01004286] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Background/Aim: Prostate cancer (PCa) is the most frequent cancer found in males worldwide. The aim of this study was to identify new biomarkers using mutated peptides for the prognosis and prediction of advanced PCa, based on proteogenomics. Materials and Methods: The tryptic peptides were analyzed by tandem mass tag-based quantitative proteomics. Proteogenomics were used to identify mutant peptides as novel biomarkers in advanced PCa. Results: Using a human database, increased levels of INTS7 and decreased levels of SH3BGRL were found to be associated with the aggressiveness of PCa. Using proteogenomics and a cancer mutation database, 70 mutant peptides were identified in PCa cell lines. Using parallel reaction monitoring, the expression of seven mutant peptides was found to be altered in tumors, amongst which CAPN2 D22E was the most significantly up-regulated mutant peptide in PCa tissues. Conclusion: Altered mutant peptides present in PCa tissue could be used as new biomarkers in advanced PCa.
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