期刊
SN APPLIED SCIENCES
卷 1, 期 6, 页码 -出版社
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.1007/s42452-019-0581-6
关键词
Graphene oxide; Alginate; Nanocomposite; Bone scaffold; Cytocompatibility; Bone tissue engineering
资金
- National Natural Science Foundation of China [31700840]
- Key Scientific Research Project of Henan Province [18B430013]
- Scientific Research Foundation of Graduate School of Xinyang Normal University [2018KYJJ48]
- Wuhan Municipal Health and Family Planning Research Fund [WX18C23]
- Nanhu Scholars Program for Young Scholars of XYNU
Porous graphene oxide (GO) nanocomposite as scaffold has attracted increasing attention in bone tissue engineering recently. In this study, GO nanosheets was modified by 2,2'-(ethylenedioxy)-diethnethiol (EDDET), and dithiol-modified GO (DT-GO) nanosheets were obtained. The results confirmed that GO nanosheets were cross-linked by EDDET, meanwhile, the distance between GO layers reduced after modification. Next, DT-GO nanosheets were further incorporated into alginate hydrogels to fabricate DT-GO/alginate (DT-GA) nanocomposite as scaffold. The prepared DT-GA nanocomposite behaved the laminar network morphology with interconnected porous structure confirmed by SEM analysis. In addition, the DT-GA nanocomposite with the concentration of DT-GO nanosheets at 16.7% showed the highest porosity value and lowest compressive strength. Furthermore, the bone marrow derived mesenchymal stem cells (BMSCs) showed a good proliferation on DT-GA nanocomposite, demonstrated that prepared nanocomposite had a good cytocompatibility, which was identified by CCK-8 assay and fluorescent microscopy images. Lastly, ALP activity analysis certified that BMSCs seeded on DT-GA nanocomposite could differentiate into osteoblastic phenotype. Above results suggest that the dithiol-modified GO/alginate nanocomposite has the potential to be applied in bone tissue engineering.
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