期刊
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
卷 71, 期 -, 页码 80-84出版社
ELSEVIER
DOI: 10.1016/j.jmbbm.2017.02.031
关键词
Erythrocytes; ICAM-4; alpha v beta 3; Surface functionalization; Viscoelasticity; Neo-Hookean
资金
- Connecticut Institute for Clinical and Translational Science (CICATS) at the University of Connecticut
- UCONN Academic Plan II
- National Science Foundation [CMMI-1235025, PHY-1205910]
- American Heart Association [12SDG12050688]
- Direct For Mathematical & Physical Scien
- Division Of Physics [1205910] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1235025] Funding Source: National Science Foundation
Current microfluidic assays, which aim at quantifying mechanical properties of sickle cell red blood cells (SSRBCs), suffer from a number of drawbacks in functionalization and flow control. Specifically, physical adsorption functionalization techniques produce inconsistent functional surfaces, and common volumetric flow pumps cannot be used to adjust the flow inside microchannels with minimal delay. We have designed an experimental setup that alleviates these complications by implementing aspiration for microchannel assembly that enables the use of most functionalization techniques and a pressure controller that allows instant and precise changes in the microchannel flow. Utilizing this setup, we have quantified SS-RBC adhesion to the integrin alpha v beta 3, a specific adhesion protein expressed on the endothelium, as well as measured the shear modulus and viscosity of the SS-RBC plasma membrane.
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