期刊
RSC ADVANCES
卷 9, 期 36, 页码 20982-20988出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9ra03203g
关键词
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资金
- National Natural Science Foundation of China [21606147, 21305079]
- Academy of Science and Technology Project of Shandong Academy of Medical Sciences [2017-55]
- key projects of industrial science and technology plan in Qiannan prefecture [(2017) 11]
- project of the Haixi Science and Technology Bureau of Qinghai Province [2017-Q4]
A simple pH fluorescent probe, N-(6-morpholino-1, 3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl) isonicotinamide (NDI), based on naphthalimide as the fluorophore and isonicotinic acid hydrazide as the reaction site was synthesized and characterized. It is useful for monitoring acidic and alkaline pH. The results of pH titration indicated that NDI exhibits obvious emission enhancement with a pK(a) of 4.50 and linear response to small pH fluctuations within the acidic range of 3.00-6.50. Interestingly, NDI also displayed strong pH-dependent characteristics with pK(a) 9.34 and linearly responded to an alkaline range of 8.30-10.50. The sensing response mechanism was confirmed by H-1 NMR and ESI-MS spectroscopy. The mechanism of the optical responses of NDI toward pH was also determined by density functional theory (DFT) calculations. In addition, NDI displayed a highly selective and sensitive response to hydrogen ions and hydroxyl ions. The probe was successfully applied to image acidic and alkaline pH value fluctuations in HeLa cells and has lysosomal targeting ability.
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