期刊
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
卷 29, 期 6, 页码 1278-1285出版社
SOC BRASILEIRA QUIMICA
DOI: 10.21577/0103-5053.20170224
关键词
biocatalysis; Fenton reaction; fluorescence; microorganism screening; PET degradation
资金
- PETROBRAS [2012/00327-7]
- CNPq (National Research Council) [140741/2013-5, 307885/2013-5]
- FAPESP-GSK [2014/50249-8]
We propose a practical, low-cost and selective fluorescence-based protocol adapted to identify polyethylene terephthalate (PET) degrading microorganisms. The microbial hydrolysis of PET nanoparticles was monitored by 2-hydroxyterephthalate, a fluorophore produced in situ after radical hydroxylation of terephthalic acid (TPA), the final hydrolysis product, by the Fenton reaction. Seven fungi presenting promising PET hydrolytic potential using the proposed microscale screening assay were identified. The strains evaluated presented a substantial increase of up to 18-fold in PET nanoparticles conversion, such as obtained by the fungus Trichoderma sp. C70, after their cultivation in a PET-enriched medium. The formation of other hydrolysis products, along with TPA, was observed using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS).
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