期刊
ANALYST
卷 144, 期 14, 页码 4162-4174出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9an00493a
关键词
-
资金
- National Natural Science Foundation of China [61675164, 61827827]
Cell-free (cf) nucleic acids are considered important and have been used as selective biomarkers. Conventional techniques for cf nucleic acid biomarker isolation from blood are generally time-consuming, complicated, and expensive. This study describes a lab-on-a-disk system equipped with newly developed immiscible filtration assisted by surface tension (IFAST), which can achieve the rapid isolation of cfDNA from whole blood. The principle of centrifugal IFAST (C-IFAST) is introduced. An arch-like channel for magnetic bead transfer in the immiscible phase is designed, which builds both a virtual water-air wall and an air-oil wall to prevent the blending of water and oil. The entire process requires less than 15 min and achieves the recovery of 65% of cfDNA from plasma and 30% from whole blood. Experiments were performed to test the validity of the chip, showing that this technique takes less time to obtain results of identical quality compared to commercial kits. The proposed C-IFAST method enables rapid and reliable cfDNA isolation from large whole blood volume (4 ml) and can potentially be used in liquid biopsy point-of-care diagnosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据