期刊
JOURNAL OF THE AMERICAN SOCIETY OF BREWING CHEMISTS
卷 75, 期 4, 页码 318-323出版社
AMER SOC BREWING CHEMISTS INC
DOI: 10.1094/ASBCJ-2017-4142-01
关键词
4-Vinyl guaiacol (4VG); Phenolic off-flavor (POF); Hydroxycinnamic acids; Fermentation
资金
- Vlaams Instituut voor Biotechnologie (VIB)
- European Molecular Biology Organization Young Investigator Programme (EMBO YIP)
- InBev-Baillet Latour Fonds
- FWO
- VLAIO [131210]
- European Research Council (ERC) Consolidator Grant [CoG682009]
- Human Frontier Science Program (HFSP) [RGP0050/2013]
Because of an increased interest in complex niche products and the ongoing quest for improved brewing efficiency and quality, the interest in novel yeasts is increasing. However, many of these nonconventional yeasts and newly developed hybrids often produce undesirable off-flavors and, in particular, are characterized by strong production of phenolic aromas such as 4-vinyl guaiacol (4VG). Here, we describe a novel highthroughput, low-cost absorbance-based screening method that allows quick determination of the phenolic off-flavor (POF) production capacity of yeasts. We show that this method correlates well with the standard, more laborious GC-based methods and that it can be used to assess POF production of many different strains in parallel with minimal hands-on labor. This new method therefore opens exciting new routes to select rare variants or mutations that combine desirable phenotypes without showing unwanted production of POFs such as 4VG.
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