期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 28, 期 10, 页码 2217-2222出版社
SPRINGER
DOI: 10.1007/s13361-017-1728-6
关键词
Cryogenic vibrational spectroscopy; Mass spectrometry; Ion mobility; Glycans; Glycan analysis
资金
- Ecole Polytechnixque Federale de Lausanne
- Swiss National Science Foundation [200020_165908]
- National Institutes of Health [R01 GM103725]
- Indiana University
- Swiss National Science Foundation (SNF) [200020_165908] Funding Source: Swiss National Science Foundation (SNF)
The structural characterization of glycans by mass spectrometry is particularly challenging. This is because of the high degree of isomerism in which glycans of the same mass can differ in their stereochemistry, attachment points, and degree of branching. Here we show that the addition of cryogenic vibrational spectroscopy to mass and mobility measurements allows one to uniquely identify and characterize these complex biopolymers. We investigate six disaccharide isomers that differ in their stereochemistry, attachment point of the glycosidic bond, and monosaccharide content, and demonstrate that we can identify each one unambiguously. Even disaccharides that differ by a single stereogenic center or in the monosaccharide sequence order show distinct vibrational fingerprints that would clearly allow their identification in a mixture, which is not possible by ion mobility spectrometry/mass spectrometry alone. Moreover, this technique can be applied to larger glycans, which we demonstrate by distinguishing isomeric branched and linear pentasaccharides. The creation of a database containing mass, collision cross section, and vibrational fingerprint measurements for glycan standards should allow unambiguous identification and characterization of these biopolymers in mixtures, providing an enabling technology for all fields of glycoscience.
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