期刊
FOOD & FUNCTION
卷 10, 期 6, 页码 3491-3501出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8fo02557f
关键词
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资金
- National 863 Program [2013AA102206-2]
Amelioration of oxidative stress has been the main approach to improve neurodegenerative disorders. In the present study, a walnut peptide with a strong capacity of scavenging reactive oxygen species (ROS) was purified and identified as EVSGPGLSPN by SEC, RP-HPLC, and HPLC-MS/MS. Treatment with EVSGPGLSPN could significantly (P < 0.05) reduce ROS generation, and increase cell viability, and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-px) activities in a dose-dependent manner in hydrogen peroxide induced PC12 cells. Western blot and immunofluorescence analysis showed that EVSGPGLSPN suppressed the expression of IKK beta and p65 to inhibit NF-kappa B pathway activation, attenuating the neurotoxic cascade by overexpression of IL-1 beta and TNF-alpha. Moreover, EVSGPGLSPN inhibited apoptosis by suppressing the expression of cytochrome C, caspase-9, caspase-3, and PARP. Additionally, it also up-regulated the expression of p-CREB and synaptophysin in oxidatively damaged PC12 cells. Thus, EVSGPGLSPN may protect against hydrogen peroxide induced neurotoxicity by enhancing the activity of antioxidant enzymes and blocking the NF-kappa B/caspase pathways.
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