期刊
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
卷 70, 期 22, 页码 2778-2790出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jacc.2017.09.1107
关键词
left stellate ganglion neural silencing; optogenetics; ventricular arrhythmia
资金
- National Nature Science Foundation of China [81530011, 81570463, 81600395]
- Fundamental Research Funds of Wuhan City [2016070204010134]
- Natural Science Foundation of Hubei Province [2016CFA065, 2016CFA048, 2016CFB621]
BACKGROUND Studies have shown that left stellate ganglion (LSG) suppression protects against ventricular arrhythmias (VAs). Optogenetics is a novel technique to reversibly regulate the activity of the targeted neurons.& para;& para;OBJECTIVES This study aimed to investigate whether an optogenetically silenced LSG could protect against VAs induced by myocardial ischemia.& para;& para;METHODS Adeno-associated virus (AAV) was used as the vector to deliver ArchT, an inhibitory light-sensitive opsin, to the LSG neurons. Twenty male beagles were randomized into the optogenetics group (n = 10, AAV2/9-CAG-ArchT-GFP microinjected into LSG) and control group (n = 10, AAV2/9-CAG-GFP microinjected into LSG). After 4 weeks, the LSG function and neural activity, heart rate variability, ventricular action potential duration, and effective refractory period were measured in the absence or presence of a light-emitting diode illumination (565 nm). Myocardial ischemia was induced by left anterior coronary artery ligation and 1 h of electrocardiography was recorded for VAs analysis.& para;& para;RESULTS ArchT was successfully expressed in alt dogs. Transient light-emitting diode illumination significantly suppressed the LSG function, LSG neural activity, and sympathetic nerve indices of heart rate variability as well as prolonged left ventricular effective refractory period and APD90 only in the optogenetics group. Thirty-minute illumination further enhanced these changes in the optogenetics group. Importantly, all of these changes returned to baseline within 2 h after illumination was turned off. Moreover, the ischemia-induced VAs were significantly suppressed by illumination only in the optogenetics group.& para;& para;CONCLUSIONS Optogenetic modulation could reversibly inhibit the neural activity of LSG, thereby increasing electrophysiological stability and protecting against myocardial ischemia-induced VAs. (C) 2017 by the American College of Cardiology Foundation.
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