期刊
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
卷 317, 期 1, 页码 F107-F115出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajprenal.00385.2018
关键词
ABT-702; adenosine kinase; cisplatin nephrotoxicity
资金
- National Natural Science Foundation of China [81670628, 81300573, 81530023]
- Natural Science Foundation of Jiangsu Province [BK20131030, BK20141503]
- China Scholarship Council [201608320124]
- Chinese Society of Nephrology Grant [17010060675]
- Clinic Research Center of Jiangsu Province [BL2014080]
- Priority Academic Program Development of Jiangsu Higher Education Institution
Numerous studies have demonstrated that several mechanisms, including oxidative stress, DNA damage. and inflammatory responses, arc closely linked to cisplatin-induced nephrotoxicity. Adenosine, emerging as a key regulatory molecule, is mostly protective in the pathophysiology of inflammatory diseases. A previous study showed that some of the adenosine receptors led to renal protection against ischemia-reperfusion injury. However, these adenosine receptor agonists lack a useful therapeutic index due to cardiovascular side effects. We hypothesized that inhibition of adenosine kinase (ADK) might exacerbate extracellular adenosine levels to reduce cisplatin-induced renal injury. In the present study. pretreatment with the ADK inhibitor ABT-702 could markedly attenuate cisplatin-induced acute kidney injury, tubular cell apoptosis, oxidative stress, and inflammation in the kidneys. Consistent with in vivo results, inhibition of ADK suppressed cisplatin-induced apoptosis, reactive oxygen species production. and inflammation in HK2 cells. Additionally, the protective effect of ADK inhibition was abolished by A(1) or A(2B) adenosine receptor antagonist and enhanced by A(2A) or A(3) adenosine receptor antagonist. Collectively, the results suggest that inhibition of ADK might increase extracellular adenosine levels. which inhibited cisplatin-induced oxidative stress and inflammation via A(1 )and A(2B )adenosine receptors, finally suppressing cisplatin-induced cell apoptosis. Pharmacological therapies based on ADK will be of potential use in therapy of cisplatin-induced nephrotoxicity.
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