Enzyme-Instructed Assembly and Disassembly Processes for Targeting Downregulation in Cancer Cells

Cancer cells differ from normal cells in both gain of 'functions (i.e., upregulation) and loss of functions (i.e., downregulation). While it is common to suppress gain of function for chemotherapy, it remains challenging to target-downregulation in-cancer cells. Here we show the combination of-enzyme-instructed assembly and disassembly to-target downregulation in cancer cells by designing peptidic precursors as the substrates of both carboxylesterases (CESs) and alkaline phosphatases (ALPs). The precursors turn into self-assembling molecules to form nanofibrils upon dephosphorylation by ALP, but CES-catalyzed cleavage of the ester bond on the Molecules results in disassembly of the nanofibrils. The precursors selectively inhibit the cancer :cells that downregulate CES (e.g., OVSAHO) but are innocuous to a hepatocyte that overexpresses CES (HepG2), while the two cell linos exhibit comparable ALP activities. This work illustrates a potential approach for the development of chemotherapy via targeting downregulation (or loss of functions) in cancer cells.