期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 140, 期 1, 页码 2-5出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.7b08518
关键词
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资金
- National Institutes of Health [GM079359, NSF 1645215]
- National Key Scientific Program of China [2011CB911000, 2013CB933701]
- NSFC [21325520, 21521063, 21327009]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM079359] Funding Source: NIH RePORTER
Enhanced targeted gene transduction by AAV2 vectors is achieved by linking the vector to multiple sgc8 aptamers, which are selective for cell membrane protein PTK7. Aptamer molecules are conjugated to multiple sites on a DNA dendrimer (G-sgc8), which is then linked to AAV2 via a dithiobis(succinimidyl propionate) cross-linker containing a disulfide group, which can facilitate the release of AAV2 vectors by reaction with the reduced form of intracellular gluta-thione. The G-sgc8-AAV2 vectors showed a 21-fold enhancement in binding affinity and an enhanced ability to protect sgc8 aptamers against nuclease degradation to cells expressing PTK7 compared to single aptamer-AAV2 conjugates. The transduction efficiency was tested by loading AAV2 with the gene for green fluorescent protein. Therefore, this modified recombinant vector is an attractive and promising tool for targeted biomedical applications.
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