期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 139, 期 20, 页码 7030-7035出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.7b02572
关键词
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资金
- NIH NIGMS R35 [1R35GM119483]
- Pew Biomedical Scholar Program [29634]
- UMN
We present a robust strategy to covalently link proteins and DNA using HUH-endonuclease domains as fusion partners (HUH-tags). We show that HUH-tags react robustly with specific sequences of unmodified single-stranded DNA, and we have identified five tags that react orthogonally with distinct DNA sequences. We demonstrate the versatility of HUH-tags as fusion partners in Cas9-mediated gene editing and the construction of doubly DNA-tethered proteins for single-molecule studies. Finally we demonstrate application to cellular imaging in live and fixed cells.
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