期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
卷 15, 期 8, 页码 1571-1581出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.34211
关键词
Caffeine; NLRP3 inflammasome; MAPK; NF-kappa B; A2aR; THP-1 macrophages
资金
- Shanghai Municipal Science and Technology Commission [134119a0500]
- Key Developing Subject Program from Shanghai Municipal Commission of Health and Family Planning [2016ZB0102]
Excessive inflammation induced by various risk factors is associated with the development of bronchopulmonary dysplasia (BPD). Caffeine exerts potent anti-inflammatory effects as a clinical preventive medicine for BPD. Recently, NLRP3 inflammasome activation has been demonstrated to be essential for the pathogenesis of BPD. In the present study, we aimed to investigate the effects of caffeine on NLRP3 inflammasome activation in LPS-induced THP-1 macrophages and to explore the underlying the detailed mechanism. We found that caffeine significantly reduced NLRP3 expression, ASC speck formation, and caspase 1 cleavage and therefore decreased IL-1 beta and IL-18 secretion in THP-1 macrophages. Caffeine also markedly decreased the phosphorylation levels of MAPK and NF-kappa B pathway members, further suppressing the translocation of NF-kappa B in THP-1 macrophages. Moreover, silencing of the caffeine-antagonized adenosine A2a receptor (A2aR) significantly decreased cleaved caspase 1 expression in THP-1 macrophages by reducing ROS production. Given these findings, we conclude that caffeine inhibits NLRP3 inflammasome activation by suppressing MAPK/NF-kappa B signaling and A2aR-associated ROS production in LPS-induced THP-1 macrophages.
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