期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 139, 期 4, 页码 1408-1411出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.6b12165
关键词
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资金
- Biotechnology and Biological Sciences Research Council (BBSRC)
- GlaxoSmithKline (GSK) [BB/K00199X/1, BB/L015048/1, BB/M017702/1]
- Royal Society
- Biotechnology and Biological Sciences Research Council [BB/M017702/1, BB/M029034/1, BB/L015048/1, E20061/2, BB/M028836/1, BB/L002655/1, BB/K00199X/1] Funding Source: researchfish
- BBSRC [BB/L002655/1, BB/M028836/1, BB/M029034/1, BB/L015048/1, BB/K00199X/1, BB/M017702/1] Funding Source: UKRI
Screening of bacterial colonies to identify new biocatalytic activities is a widely adopted tool in biotechnology, but is constrained by the requirements for colorimetric or tag-based detection, methods. Herein we report a label-free screening platform for biotransformations in live colonies using desorption electrospray ionization coupled with ion mobility mass spectrometry imaging (DiBT-IMMS). The screening method is demonstrated for both ammonia lyases and P450 monooxygenases expressed within live bacterial colonies and is shown to enable multiplexing of enzyme variants and substrate libraries simultaneously.
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