期刊
BIO-PROTOCOL
卷 9, 期 15, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.3326
关键词
Candida albicans; Subdermal infection; Hypoxia; IVIS; Mycology
类别
资金
- Swedish Research Council [VR-M 2014-02281, 2017-01681]
- Kempe Foundation [SMK-1453]
- J.C. Kempes Minnes Stipendiefond
- Arneska Stiftelse
- Helge ax: son Johnsons Stiftelse
- Swedish Research Council [2017-01681] Funding Source: Swedish Research Council
- Forte [2017-01681] Funding Source: Forte
- Vinnova [2017-01681] Funding Source: Vinnova
Candida albicans is a leading human fungal pathogen that uses several metabolic adaptations to escape immune cells and causes systemic disease. Here, we describe a protocol for measuring one of these adaptations, the ability to thrive in hypoxic niches. Hypoxia was generated after successful subdermal infection with C. albicans in a murine infection model. Hypoxia was measured using a fluorescent dye for carbonic anhydrase 9, a host enzyme active under hypoxic conditions. Emitted fluorescence was subsequently quantified using an IVIS system. This protocol was optimized for the use in subdermal infection in mice but has the potential to be adapted to other models of fungal infection.
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