期刊
GENOME BIOLOGY
卷 20, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13059-019-1783-3
关键词
CRISPR; sgRNA design; Genomics; Genome surgery; Genome editing; Computational biology
资金
- Gladstone Institutes
- Claire Giannini Fund
- UCSF Discovery Fellows Program
- National Institutes of Health [UM1HL098179, P01HL089707, R01MH109907, U01HL100406, R01HL130533, 1R01EY028249]
The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Target sites might carry genetic variations that are not distinguishable by sgRNA designing tools based on one reference genome. AlleleAnalyzer is an open-source software that incorporates single-nucleotide variants and short insertions and deletions to design sgRNAs for precisely editing 1 or multiple haplotypes of a sequenced genome, currently supporting 11 Cas proteins. It also leverages patterns of shared genetic variation to optimize sgRNA design for different human populations. AlleleAnalyzer is available at .
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