期刊
APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
卷 17, 期 4, 页码 7249-7263出版社
CORVINUS UNIV BUDAPEST
DOI: 10.15666/aeer/1704_72497263
关键词
carbon quantum dots; pig liver; bacterial staining; in vivo bio-imaging; MnO4-
In this paper, novel fluorescent carbon quantum dots (CQDs) were successfully prepared from pig liver by a simple one-step hydrothermal method. The prepared CQDs were characterized using TEM, XRD, XPS, FT-IR, UV-Vis, and fluorescence spectroscopy. It was found that the CQDs were uniformly distributed in size and exhibited good excitation dependence and excellent photostability. Fluorescent probe was used for detection of permanganate. This paper also studied the fluorescent staining performance of pig liver-based CQDs on four live/dead bacteria to evaluate the low toxicity and application prospects of the CQDs. Under the optimum experimental conditions, the probe showed a good linear range (R-2 = 0.9967) and a low detection limit (0.06 mu M) in the concentration range of 0.1-50 mu M. The pig liver based-CQDs were proved to be a low toxicity fluorescent dye. These attractive properties indicated that the new CQDs could adapt to various complex pH environments, and thus had extensive prospect and promising application for detection of permanganate in complex environmental samples.
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