期刊
COMMUNICATIONS BIOLOGY
卷 2, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s42003-019-0550-z
关键词
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资金
- NSF [CBET-1752106]
- American Cancer Society [IRG-67-003-50]
- Pelotonia Junior Investigator Award, NIH [R01HL141941]
- Ohio State University Materials Research Seed Grant Program - Center for Emergent Materials, an NSF-MRSEC [DMR-1420451]
- Center for Exploration of Novel Complex Materials
- Institute for Materials Research
- Office of the Assistant Secretary of Defense for Health Affairs, through the Lung Cancer Research Program [W81XWH-17-1-0233]
- Department of Mechanical and Aerospace Engineering at The Ohio State University
Interactions between cells and their environment influence key physiologic processes such as their propensity to migrate. However, directed migration controlled by extrinsically applied electrical signals is poorly understood. Using a novel microfluidic platform, we found that metastatic breast cancer cells sense and respond to the net direction of weak (similar to 100 mu V cm(-1)), asymmetric, non-contact induced Electric Fields (iEFs). iEFs inhibited EGFR (Epidermal Growth Factor Receptor) activation, prevented formation of actin-rich filopodia, and hindered the motility of EGF-treated breast cancer cells. The directional effects of iEFs were nullified by inhibition of Akt phosphorylation. Moreover, iEFs in combination with Akt inhibitor reduced EGF-promoted motility below the level of untreated controls. These results represent a step towards isolating the coupling mechanism between cell motility and iEFs, provide valuable insights into how iEFs target multiple diverging cancer cell signaling mechanisms, and demonstrate that electrical signals are a fundamental regulator of cancer cell migration.
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