期刊
JOURNAL OF SURFACTANTS AND DETERGENTS
卷 20, 期 5, 页码 1141-1151出版社
WILEY
DOI: 10.1007/s11743-017-1986-0
关键词
Glycolipid; Bacillus licheniformis; Thermogravimetric analysis; Prostate cancer
资金
- Department of Science and Technology, INSPIRE (DST, INSPIRE) Govt. of India [IF130678]
A highly potent glycolipid was isolated from a crude oil-contaminated soil bacterium Bacillus licheniformis SV1 (NCBI GenBank Acc. No. KX130852) when grown on a modified mineral salt medium supplemented with 2% oleic acid. The maximum reduction in surface tension of cell-free broth from 71 +/- 0.812 to 25.919 +/- 0.984 mN/m with 89 +/- 0.346% emulsification activity was recorded after 120 h of growth. Glycolipid was purified using chromatographic techniques and the presence of aliphatic chain (C-H stretch) and OH-band was revealed by NMR, GC-MS and FTIR analysis. Stability of glycolipid up to 250 A degrees C and its complete decomposition at 507 A degrees C was recorded by thermogravimetric analysis (TGA). MTT assay (IC50 = 0.473 +/- 0.048 mg/ml) along with 4',6-diamidino-2-phenylindole (DAPI) and acridine orange and ethidium bromide (AO/EtBr) staining validated that glycolipid induces cell apoptosis in human prostate cancer cell line (PC-3). Furthermore, potent anti-cancerous compounds such as 9-octadecanoic acid (22.55%), linoleic acid methyl ester (2.2%) and palmitic acid (1.18%) were also detected in the GC-MS spectra of the purified glycolipid.
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