期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 197, 期 3, 页码 220-226出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2016.11.002
关键词
Cryo electron microscopy; Sample preparation; Microfluidics
资金
- Swiss Nanoscience Institute (SNI) [P1201]
- Swiss National Science Foundation (SNF) [200021_162521]
- Swiss National Science Foundation (SNF) [200021_162521] Funding Source: Swiss National Science Foundation (SNF)
We present a sample preparation method for cryo-electron microscopy (cryo-EM) that requires only 320 nL of sample to prepare a cryo-EM grid, depending on the protocol used. The sample is applied and spread on the grid by a microcapillary. The procedure does not involve any blotting steps, and realtime monitoring allows the water film thickness to be assessed and decreased to an optimum value prior to vitrification. We demonstrate that the method is suitable for high-resolution cryo-EM and will enable alternative electron microscopy approaches, such as single-cell visual proteomics. (C) 2016 The Author(s). Published by Elsevier Inc.
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