期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 197, 期 1, 页码 37-42出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2016.03.011
关键词
Mechanotransduction; Molecular force microscopy; FRET-based tension sensors; Single-molecule spectroscopy
资金
- Boehringer Ingelheim Fonds PhD fellowship
- International Max Planck Research School for Molecular and Cellular Life Sciences (IMPRS-LS)
- Nanosystems Initiative Munich (NIM)
- Max Planck Forderstiftung
- German Research Council through the Collaborative Research Centre [SFB863]
- German Research Council [GR3399/5-1]
The ability of cells to sense and respond to mechanical forces is crucial for a wide range of developmental and pathophysiological processes. The molecular mechanisms underlying cellular mechanotransduction, however, are largely unknown because suitable techniques to measure mechanical forces across individual molecules in cells have been missing. In this article, we highlight advances in the development of molecular force sensing techniques and discuss our recently expanded set of FRET-based tension sensors that allows the analysis of mechanical forces with piconewton sensitivity in cells. In addition, we provide a theoretical framework for the design of additional tension sensor modules with adjusted force sensitivity. (C) 2016 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据