期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 206, 期 1, 页码 1-11出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2017.11.015
关键词
Solid-state NMR; BAM; MAS; E. coli; Membrane protein complex
资金
- Netherlands Organization for Scientific Research (NWO) [700.26.121, 700.10.443]
- iNEXT [653706]
- uNMR-NL
- NWO [184.032.207]
Significant progress has been made in obtaining structural insight into the assembly of the beta-barrel assembly machinery complex (BAM). These crystallography and electron microscopy studies used detergent as a membrane mimetic and revealed structural variations in the central domain, BamA, as well as in the lipoprotein BamC. We have used cellular solid-state NMR spectroscopy to examine the entire BamABCDE complex in native outer membranes and obtained data on the BamCDE subcomplex in outer membranes, in addition to synthetic bilayers. To reduce spectral crowding, we utilized proton-detected experiments and employed amino-acid specific isotope-labelling in (C-13, C-13) correlation experiments. Taken together, the results provide insight into the overall fold and assembly of the BAM complex in native membranes, in particular regarding the structural flexibility of BamC in the absence of the core unit BamA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据