期刊
DNA AND CELL BIOLOGY
卷 34, 期 1, 页码 29-36出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/dna.2014.2522
关键词
-
资金
- National Science and Technology Major Project [2012ZX10002006]
- Key Technologies R&D Program of Zhejiang Province [2012C03SA170003]
- Hangzhou Key Technologies RD Program [20122513A49]
microRNAs are endogenous noncoding RNA molecules of similar to 22 nucleotides that regulate gene function by modification of target mRNAs. Due to tissue specific of miR-133a and miR-1/206 for skeletal muscles, we investigated the role of miR-133a and miR-1/206 in promoting the differentiation of the C2C12 cells. The results show that directly transfecting mature miR-133a, miR-1/206, or combinations (miR-1 and miR-206, miR-1 and miR-133a, and miR-133a and miR-206) into C2C12 cells, respectively, for 5 days induces formation of myogenic progenitor cells. Overexpression of miR-133a and miR-206 in C2C12 cells greatly improved multinucleated myotube formation. microRNA-133a (miR-133a) is highly expressed during human muscle development. Using bioinformatics, we identified one putative miR-133a binding site within the 3 '-untranslated region of the mouse Foxl2 mRNA. The expression of Foxl2 was shown to be downregulated by subsequent western blot analysis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据