期刊
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 172, 期 -, 页码 149-159出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jsbmb.2017.07.007
关键词
Aucubin; Hindlimb ischemia; Angiogenesis; ER beta; VEGF; Peripheral vascular disease
资金
- Natural Science Foundation of Tianjin Municipal Government [16JCZDJC36300]
- Program of International S & T Cooperation Project of China [2015DFA30430]
- National Natural Science Foundation of China [81603329, 81125024]
- Program for New Century Excellent Talents in University of Ministry of Education of China [NCET-13-0935]
- Program for Changjiang Scholars and Innovative Research Team in University, PCSIRT [IRT1276]
Aucubin (AU) is an iridoid glycoside that has been shown to display estrogenic properties and has various pharmacological effects. Herein, we described the angiogenic properties of AU. In the study, hindlimb ischemia was induced by ligation of femoral artery on the right leg of ovariectomized mice. AU treatment significantly accelerated perfusion recovery and reduced tissue injury in mice muscle. Quantification of CD31-positive vessels in hindlimb muscles provided evidences that AU promoted angiogenesis in peripheral ischemia. In addition, results from quantitative PCR and western blot suggested AU induced angiogenesis via vascular endothelial cell growth factor (VEGF)/Akt/endothelial nitric oxide synthase (eNOS) signaling pathway. More interestingly, AU's angiogenic effects could be completely abolished in estrogen receptor beta (ER beta) knockout mice. In conclusion, the underlying mechanisms were elucidated that AU produced pro-angiogenic effects through ERD-mediated VEGF signaling pathways. These results expand knowledge about the beneficial effects of AU in angiogenesis and blood flow recovery. It might provide insight into the ER beta regulating neovascularisation in hindlimb ischemia and identify AU as a potent new compound used for the treatment of peripheral vascular disease.
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