期刊
CHEMICAL SCIENCE
卷 10, 期 34, 页码 7852-7858出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9sc02021g
关键词
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资金
- Australia National Health and Medical Research Council [NHMRC APP1163786]
- Scientia Fellowship program at UNSW
- Australian Research Council (ARC) [DP190101008]
- China Scholarship Council
- Australian Government Research Training Program Scholarship
- Australian Research Council Discovery Early Career Researcher Award [ARC DECRA DE170100068]
Despite the promise of metal-organic frameworks (MOFs) as functional matrices for enzyme stabilization, the development of a stimulus-responsive approach to induce a multi-enzyme cascade reaction in MOFs remains a critical challenge. Here, a novel method using peptide-induced super-assembly of MOFs is developed for programmed enzyme cascade reactions on demand. The super-assembled MOF particles containing different enzymes show remarkable 7.3-fold and 4.4-fold catalytic activity enhancements for the two-enzyme and three-enzyme cascade reactions, respectively, as compared with the unassembled MOF nanoparticles. Further digestion of the coiled-coil forming peptides on the MOF surfaces leads to the MOF superstructure disassembly and the programmed enzyme cascade reaction being switchedoff. Research on these stimuli-responsive materials with controllable and predictable biocatalytic functions/ properties provide a concept to facilitate the fabrication of next-generation smart materials based on precision chemistry.
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