期刊
JOURNAL OF THE CERAMIC SOCIETY OF JAPAN
卷 127, 期 9, 页码 636-641出版社
CERAMIC SOC JAPAN-NIPPON SERAMIKKUSU KYOKAI
DOI: 10.2109/jcersj2.19114
关键词
TiO2 surface; Surface potential; Cellular response; Fibronectin; Immunogold-labeling technique; High-speed atomic force microscopy
资金
- Kibankenkyu C grant [16K06786]
- Japan Society for the Promotion of Science (JSPS)
MC3T3-E1 cell differentiation is more pronounced after 14 days incubation on charged nitrogen-doped TiO2 surfaces compared to on an untreated, neutral Ti surface. The protein fibronectin (Fn) was detected by an immunogold-labeling technique and Ca and P were detected by time-of-flight secondary ion mass spectrometry. Both techniques revealed that an adhesive protein such as Fn adsorbs equally on negatively-charged, positively-charged, and untreated Ti surfaces in culture medium. However, the adsorption of Ca and P was only detected on charged nitrogen-doped TiO2 surfaces. The enhanced adsorption of inorganic ions and Fn is probably responsible for promoting initial stage of osteoblast differentiation. The conformation of adsorbed Fn was observed by high-speed atomic force microscopy and found to be in the side-on orientation on the positively-charged surface. This finding may help elucidate the relation between Fn conformation and cell activity on surface potential-controlled nitrogen-doped TiO2 surfaces in future. (C) 2019 The Ceramic Society of Japan. All rights reserved.
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