期刊
JOURNAL OF SEPARATION SCIENCE
卷 40, 期 21, 页码 4222-4233出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201700549
关键词
antidepressant drugs; biological fluids; gas chromatography-mass spectrometry; magnetic micro-solid-phase extraction; magnetite
资金
- Ministry of Education Malaysia [Q.J130000.2526.02H29, 5524938]
A new facile magnetic micro-solid-phase extraction coupled to gas chromatography and mass spectrometry detection was developed for the extraction and determination of selected antidepressant drugs in biological fluids using magnetite-MCM-41 as adsorbent. The synthesized sorbent was characterized by several spectroscopic techniques. The maximum extraction efficiency for extraction of 500 mu g/L antidepressant drugs from aqueous solution was obtained with 15 mg of magnetite-MCM-41 at pH 12. The analyte was desorbed using 100 mu L of acetonitrile prior to gas chromatography determination. This method was rapid in which the adsorption procedure was completed in 60 s. Under the optimized conditions using 15 mL of antidepressant drugs sample, the calibration curve showed good linearity in the range of 0.05-500 mu g/L (r(2) = 0.996-0.999). Good limits of detection (0.008-0.010 mu g/L) were obtained for the analytes with good relative standard deviations of <8.0% (n = 5) for the determination of 0.1, 5.0, and 500.0 mu g/L of antidepressant drugs. This method was successfully applied to the determination of amitriptyline and chlorpromazine in plasma and urine samples. The recoveries of spiked plasma and urine samples were in the range of 86.1-115.4%. Results indicate that magnetite micro-solid-phase extraction with gas chromatography and mass spectrometry is a convenient, fast, and economical method for the extraction and determination of amitriptyline and chlorpromazine in biological samples.
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