期刊
JOURNAL OF SEPARATION SCIENCE
卷 40, 期 13, 页码 2791-2799出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201700116
关键词
aristolochic acid I; herbal medicines; solid-phase extraction; surface molecularly imprinted polymers
资金
- National Natural Science Foundation of China [81573391]
- National Key Projects of China [81227802]
- Science and Technology Projects of Shaanxi Social Development [2016SF-159]
- Shaanxi Province Administration of Traditional Chinese Medicine Foundation [15ZY027]
In this study, surface molecularly imprinted polymers were prepared as the selective sorbents for separation of aristolochic acid I in herbal medicine extracts by a facile approach. A less toxic dummy template, ofloxacin, was used to create specific molecule recognition sites for aristolochic acid I in the synthesized polymers. The polymers were characterized by Fourier-transfer infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, elemental analysis, and nitrogen adsorption-desorption test. The adsorption capacity was calculated using adsorption kinetics, selectivity, and recycling experiments. The obtained polymers exhibited high thermostability, fast equilibrium time, and excellent binding ability. Subsequently, the polymers applied as the solid-phase extraction absorbent was proposed and used for the enrichment and analysis of aristolochic acid I in herbal plants. The result showed that the aristolochic acid I was enriched up to 16 times after analysis by using high-performance liquid chromatography. The good linearity for aristolochic acid I was obtained in the range of 0.1-200 mu g/mL (R-2 = 0.9987). The recovery and precision values were obtained (64.94-77.73%, RSDs% = 0.8%, n = 3) at three spiked concentration levels. This work provided a promising method for selective enrichment, extraction, and purification of aristolochic acid I from complex herbal plants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据