期刊
JOURNAL OF SEPARATION SCIENCE
卷 40, 期 5, 页码 1209-1217出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201601130
关键词
maslinic acid; microwave-assisted extraction; oleanolic acid; olive skin
资金
- Agricultural Research Institute of Spain (INIA)
- European Social Fund
- Consejeria de Innovacion, Ciencia y Empresa of the Junta de Andalucia [P11-FQM-7372]
Microwave-assisted extraction is compared with a more classical technique, Soxhlet extraction, to determine the content of triterpene acids in olive skins. The samples used in their original unmilled state and milled were extracted with ethyl acetate or methanol as solvents. The optimized operating conditions (e.g., amount and type of solvent, and time and temperature of extractions) to attain the better extraction yields have been established. For the identification and quantitation of the target compounds, an ultra high performance liquid chromatography with tandem mass spectrometry method was employed. The best results were achieved using the microwave-assisted extraction technique, which was much faster than the Soxhlet extraction method, and showed higher efficiency in the extraction of the triterpenic acids (oleanolic and maslinic).
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