期刊
JOURNAL OF SEPARATION SCIENCE
卷 41, 期 2, 页码 582-589出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201700575
关键词
color values; Crataegi Fructus; fingerprint chromatogram; spectrophotometry
资金
- National Natural Science Foundation of China [81573604, 30701108, 81673462, 81473293, 91540119]
- Key Projects of Natural Science Foundation of Jiangsu Province for University [13KJA360003]
- Preliminary Research Foundation of Nanjing University of Chinese Medicine [12XYY06]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Crataegi Fructus and its processed products have been used as a traditional medicine for a long time, and numerous active components are responsible for their curative effects. However, a comprehensive and fast method for the quality control of its processed products is still lacking. In this study, two analytical methods based on color measurements and fingerprint analysis are established. In the color measurements, the color values of the peel and flesh of Crataegi Fructus were evaluated spectrophotometrically. Based on the results, a color reference range was established using percentiles, and the standard color difference value was established using the median color values. Then, the color values of Crataegi Fructus and its processed products were analyzed using Bayes linear discriminant analysis and mathematical functions were built in order to predict the degree of processing. Moreover, high-performance liquid chromatography fingerprint analysis was performed on a Hibar C18 column, and a high-performance liquid chromatography fingerprint pattern was obtained, from which nine peaks were identified. Chemometric methods were successfully applied to differentiate raw and processed Crataegi Fructus.
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