期刊
ENEURO
卷 6, 期 4, 页码 -出版社
SOC NEUROSCIENCE
DOI: 10.1523/ENEURO.0140-19.2019
关键词
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资金
- Spanish Ministry of Economy and Competitiveness [SAF 2015-66603-P]
- Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas (CIBERNED, Spain) [CB06/05/0066]
- Alzheimer's Association [ZEN-15-321663]
- European Union's Horizon 2020 research and innovation program [785907, 2]
- FPU contract from the Spanish Ministry of Education, Culture and Sports (MECD) [FPU 14/02245]
The transentorhinal cortex (TEC) is an obliquely oriented cortex located in the medial temporal lobe and, together with the entorhinal cortex, is one of the first affected areas in Alzheimer's disease (AD). One of the most widely accepted hypothesis is that synaptopathy (synaptic alterations and loss) represents the major structural correlate of the cognitive decline observed in AD. However, very few electron microscope studies are available; the most common method to estimate synaptic density indirectly is by counting -at the light microscopic level- immunoreactive puncta using synaptic markers. To investigate synaptic morphology and possible alterations related to AD, a detailed three-dimensional (3D) ultrastructural analysis using focused ion beam/scanning electron microscopy (FIB/SEM) was performed in the neuropil of layer II of the TEC in human brain samples from non-demented subjects and AD patients. Evaluation of the proportion and shape of asymmetric and symmetric synapses (AS and SS, respectively) targeting spines or dendritic shafts was performed using 3D reconstructions of every synapse. The 3D analysis of 4722 synapses revealed that the preferable targets were spine heads for AS and dendritic shafts for SS, both in control and AD cases. However, in AD patients, we observed a reduction in the percentage of synapses targeting spine heads. Regarding the shape of synapses, in both control cases and AD samples, the vast majority of synapses had a macular shape, followed by perforated or horseshoe-shaped synapses, with fragmented synapses being the least frequent type. Moreover, comparisons showed an increased number of fragmented AS in AD patients.
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