期刊
BMB REPORTS
卷 52, 期 8, 页码 475-481出版社
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
DOI: 10.5483/BMBRep.2019.52.8.149
关键词
CRISPR; DNA double-strand break; Genome editing; HDR; NHEJ
资金
- Chung Yang, Cha Young Sun & Jang Hi Joo Memorial fund
- Korea university grant [K1804351]
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) of Korea [NRF-2018M3A9H3021707, NRF-2018R1D1A1B07048434, NRF-2014M3A9D5A01075128]
- National Research Foundation of Korea [2014M3A9D5A01075128, 2018M3A9H3021707] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The evolution of genome editing technology based on CRISPR (clustered regularly interspaced short palindromic repeats) system has led to a paradigm shift in biological research. CRISPR/Cas9-guide RNA complexes enable rapid and efficient genome editing in mammalian cells. This system induces double-stranded DNA breaks (DSBs) at target sites and most DNA breakages induce mutations as small insertions or deletions (indels) by non-homologous end joining (NHEJ) repair pathway. However, for more precise correction as knock-in or replacement of DNA base pairs, using the homology-directed repair (HDR) pathway is essential. Until now, many trials have greatly enhanced knock-in or substitution efficiency by increasing HDR efficiency, or newly developed methods such as Base Editors (BEs). However, accuracy remains unsatisfactory. In this review, we summarize studies to overcome the limitations of HDR using the CRISPR system and discuss future direction.
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