A study to evaluate the effect of phyto-silver nanoparticles synthesized using Oxalis stricta plant leaf extract on extracellular fungal amylase and cellulase

In the present study, an attempt has been made to evaluate the effect of plant mediated synthesized silver nanoparticles against two industrially important enzymes namely amylase and cellulase that are produced using Aspergillus niger through submerged fermentation under optimum conditions. The crude enzyme thus, obtained was incubated with the respective substrate in presence of synthesized phyto silver nanoparticles and the enzyme activity was determined with different concentration of phyto silver nanoparticles. Silver nanoparticles were fabricated using plant leaf extract of Oxalis stricta. The characterizations of the synthesized silver nanoparticles were determined Fourier transform infrared spectroscopy (FT-IR) and Scanning electron microscopy (SEM). The synthesized silver nanoparticles were found to be potentially active in enhancing the activity of fungal amylases and cellulase. Hence the study concludes that the biogenic synthesis of silver nanoparticles is a rapid, simple, cost-effective and environment benign method and these silver nanoparticles may have potential application in various processes that requires amylolytic and cellulolytic activity at commercial or industrial levels. (C) 2019 Elsevier Ltd. All rights reserved.